Biological characteristics and classification of thermophilic actinomycetes showed extracellular hydrolytic enzymes producing ability isolated from compost

Compost is a highly humified organic fertilizer, rich in nutrients and source of variety aerobic microorganisms, including actinomycetes, which develop response to different levels temperature, humidity, oxygen pH. Microbes growing on the compost are believed have ability produce extracellular hydrolytic enzymes. The purpose this study was determine thermophilic actinomycetes XM21 isolated from producing enzymes, namely cellulase, amylase, protease, lipase. confirmation tests enzymes-producing were conducted by inoculating microbes into media containing cellulose, starch, gelatin tween 80, using method disc diffusion. results showed that strain capable enzymes producing, such as Strain can grow well with high cellulase activity wide range temperature between 30-55oC, optimum at 45oC. media, utilized carbon sources pH 5-10, salinity 0-5%. On agar plate, has white aerial mycelia, mature spore chains appeared spirals, moderately long, bearing 10 35 spores each. Based biological characteristics phylogenetic analysis 16S rDNA, it be concluded close Streptomyces flavovariabilis (98, 12%), hence identified XM21.